Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Glioblastoma)
Specification
Glioblastoma
Fixative
Paraformaldehyde
Permeabilization
Yes - TBS Tween 0.5%
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce TBS Super block Blocking Buffer
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 594 Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Maïté Verreault
Verified customer
投稿 May 02 2013