Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

2nd: 1/5000, 1/7000 high background
sees precipitate at bottom

trying right now same primary with other secondary
suggested to try no-primary crtl to check if problem comes from primary or secondary Ab

ANSWER:

Thank you for contacting us.
I am sorry to hear that one of these antibodies is notworking properly.

As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem comes from the primary or secondary antibody.

I look forard to hear back from and assist you in resolving this problem.

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We see multiple bands in blots using just the secondary antibody.

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

thanks for your fast reply.
The Abcam HRP antibody didn't give any signal (more than some black spots) but the same primary antibody worked nicely at the same time point with another secondary antibody.
We tried to dissolve the antibody, but as you can see on the pictures there's still remaining significant clumps.

Can you therefore, please send me the ab97051 as a replacement vial.

Best regards,

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1077750.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

we were really unhappy to find out that the antibody weordered (date: 13thof April 2012) from Abcam (order number:1070469,lot number: GR27131)have precipitated, (se attached pictures).When we anywaytried to do thewestern blot unfortunately, this didn’t work.This is extra unfortunatebecause we had to usethe antibody for a demo of western blot imagingsystem.
As you can se on the attached images the antibody forms a significant pellet (Pic_I) and when we tried to dissolve it it still large clumps in the solution (Pic_II).
We have handled the antibody according to your instruction (kepttheantibody at +4C).Please contact me to let me know how to proceed with this.
Best regards,

ANSWER:

Thank you for your email. I am sorry to hear about the inconvenience caused.
We are aware of this problem and doing our best to resolve this issue. We generally recommend our customers dissolving the lumps and using the antibody as normal, in many case we have even asked customers to centrifuge out the precipitates and for many customer the antibody has worked perfectly well. There are however inconsistencies with the complaints we receive from our customers. Although small lumps are very common with HRP conjugated however because this is a constant issue with this antibody so we are interested in collecting more feedback. Could you please provide some details about the problem;
- Does this antibody gave high background, multiple bands or no staining?
- Have you tried any sort of troubleshooting to optimize the experiment?
- Is the primary antibody fine? Have you tried the primary antibody before or with different secondary antibody.
Finally, would you like to try the antibody ab97051 or ab97069 as replacement.
I will look forward to hearing from you soon.

Estimada
Nos podeis reponer los dos viales del anticuerpo ab6721, es el anticuerpo que necesitamos. Pero si todos los del mismo lote, tienen el mismo problema dimelo para ver que hacemos.
Gracias por todo
Un saludo

ANSWER:

Gracias por tu respuesta.

En principio el precipitado amarillo presente en el vial no debería aparecer, y los viales que habéis recibido lamentablemente si lo tenían.

Puedo mandaros dos nuevos viales confiando en que no vuelva a pasar (que es lo normal), o si preferís cambiar de anticuerpo ya que el anterior no os funcionó, puedo mandaros uno distinto de las mismas características, siempre y cuando me indiquéis las categorías que os pregunté en el mail anterior, que son necesarias para poder sugeriros la mejor alternativa.

Así pues, lo dejo a vuestra elección, y quedo a la espera de que me lo indiquéis para proceder con el pedido.