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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)

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    Overview

    Product name

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin)
    IgY 抗体 (89件) 一覧

    Target species

    Chicken

    Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    Tested applications

    ELISA, IHC-P, ICC/IF, WBmore details

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C.

    Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS

    Concentration

    Concentration information loading...

    Purity

    Immunogen affinity purified

    Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

    Conjugation notes

    Biotin/ antibody protein ratio is 4:1

    Clonality

    Polyclonal

    Isotype

    IgG

    Research areas

    Secondary antibodies >> anti-Chicken >> IgY >> Biotin

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133) image (enlarge)

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab97133 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • ShowHide

      ELISA

       ELISA: 1/20000 - 1/40000...Read more →

      ELISA: 1/20000 - 1/400000.((Primary).)

    • 1 Image

      IHC-P

       IHC-P: 1/500 - 1/5000.

      IHC-P: 1/500 - 1/5000.

    • ICC/IF

       ICC/IF: 1/200 - 1/1000.

      ICC/IF: 1/200 - 1/1000.

    • ShowHide

      WB

       WB: 1/20000 - 1/400000.(...Read more →

      WB: 1/20000 - 1/400000.((Chemiluminescent).)

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) images:

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)

    Ab97133 was used at dilution 1/200 with the primary antibody ab81755 in IHC-P. See the review on ab81755.

    References for Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin) (ab97133)

    ab97133 has not yet been referenced specifically in any publications.

    Publishing research using ab97133? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"