Application
Western blot
Lane 1 is wild-type mouse ES cells expressing E-cadherin. Lane 2 is an Ecad-/- mES cell line. DECMA-1 primary antibody used (not Abcam). Secondary used at 1:5000 for 1 hour. Use of secondary at higher concentration of 1:2000 results in background staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 28 2013