Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)
Key features and details
- Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
アプリケーション
適用あり: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
免疫原
Rat IgG, whole molecule (H&L)
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標識
Cy5 ®. Ex: 650nm, Em: 667nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6565の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab6565 は 33 報の論文で使用されています。
- Saha LK et al. Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes. Nat Commun 14:1925 (2023). PubMed: 37024461
- Chang YC et al. RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication. Open Biol 13:230068 (2023). PubMed: 37607592
- Rios-Szwed DO et al. FAM111A regulates replication origin activation and cell fitness. Life Sci Alliance 6:N/A (2023). PubMed: 37793778
- Fu H & Aladjem MI DNA replication profiling by molecular combing on single DNA fibers. STAR Protoc 3:101290 (2022). PubMed: 35463463
- Moore G et al. DNA fiber combing protocol using in-house reagents and coverslips to analyze replication fork dynamics in mammalian cells. STAR Protoc 3:101371 (2022). PubMed: 35573479