Application
Immunocytochemistry/ Immunofluorescence
Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, and immunofluorescently labeled with an anti-histone H3 (phospho S10) antibody, which is expected to stain only mitotic cells. The fluorescent secondary antibody (ab150157) exhibited the correct affinity for the primary antibody with no apparent background or non-specific staining. In the "Merge" image below, the DAPI is pseudocolored red, while ab150157 is pseudocolored green.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Oct 18 2013