Goat Anti-Rabbit IgG H&L (ab182016)
Key features and details
- Goat Anti-Rabbit IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: IP, IHC-P, WB, ELISA
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
The antibody reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Mouse IgG, rat IgG, and chicken IgY, less than 2%. Human IgG, less than 7%. -
アプリケーション
適用あり: IP, IHC-P, WB, ELISAmore details -
免疫原
The details of the immunogen for this antibody are not available.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182016の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ELISA |
1/20000 - 1/200000.
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特記事項 |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ELISA
1/20000 - 1/200000. |
画像
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Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.
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Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab182016 は 8 報の論文で使用されています。
- Chen Y et al. A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer. Cell Death Dis 14:8 (2023). PubMed: 36609474
- Zhang W et al. miR-195-3p/BDNF axis regulates hypoxic injury by targeting P-ERK1/2 expression. Medicine (Baltimore) 101:e31586 (2022). PubMed: 36401373
- Chang CC et al. A Coronary Artery Disease Monitoring Model Built from Clinical Data and Alpha-1-Antichymotrypsin. Diagnostics (Basel) 12:N/A (2022). PubMed: 35741224
- Tektemur A et al. Carbamazepine-induced sperm disorders can be associated with the altered expressions of testicular KCNJ11/miR-let-7a and spermatozoal CFTR/miR-27a. Andrologia 53:e13954 (2021). PubMed: 33372325
- Bi J et al. Role of long intergenic non-protein coding RNA 01857 in hepatocellular carcinoma malignancy via the regulation of the microRNA-197-3p/anterior GRadient 2 axis. PLoS One 16:e0258312 (2021). PubMed: 34793477
- Zhang B et al. Dexmedetomidine attenuates ventilator-induced lung injury in rats by up-regulating NLRC3. Ann Palliat Med 9:2474-2484 (2020). PubMed: 32787359
- Qian H et al. Combined application of Embelin and tumor necrosis factor-related apoptosis-inducing ligand inhibits proliferation and invasion in osteosarcoma cells via caspase-induced apoptosis. Oncol Lett 15:6931-6940 (2018). PubMed: 29731867
- Chen A et al. GGNBP2 is necessary for testis morphology and sperm development. Sci Rep 7:2998 (2017). PubMed: 28592902