Goat Anti-Rabbit IgG H&L (PE) preadsorbed (ab72465)
Key features and details
- Goat Anti-Rabbit IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ELISA
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (PE) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: Flow Cyt, ELISAmore details -
吸着処理血清
Cow, Mouse more details -
免疫原
Rabbit IgG
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標識
PE. Ex: 488nm, Em: 575nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.71% Sucrose, 0.27% sodium dihydrogen phosphate, 1.09% disodium hydrogen phosphate -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
特記事項(標識)
Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | (1) |
1/500 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
|
特記事項 |
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Flow Cyt
1/500 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
画像
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Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.
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Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab72465 は 17 報の論文で使用されています。
- Ou S et al. Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling. PeerJ 11:e15894 (2023). PubMed: 37727693
- Gamal-Eldeen AM et al. Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma. Front Nutr 9:854780 (2022). PubMed: 35399691
- Gouhar SA et al. Involvement of miRNAs in response to oxidative stress induced by the steroidal glycoalkaloid α-solanine in hepatocellular carcinoma cells. Environ Toxicol 37:212-223 (2022). PubMed: 34655286
- Derkus B et al. Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv Biol (Weinh) 6:e2101317 (2022). PubMed: 35347890
- Qiang P et al. Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone. Front Immunol 13:948658 (2022). PubMed: 36148244