Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
- Conjugation: DyLight® 594. Ex: 593nm, Em: 618nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
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アプリケーション
適用あり: ICC/IF, IHC-P, Flow Cytmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details -
免疫原
Other Immunogen Type corresponding to Mouse IgG.
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標識
DyLight® 594. Ex: 593nm, Em: 618nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96881の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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特記事項 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
画像
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
ab96881 was used at dilution 1/100 for IHC analysis of FFPE human kidney. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1/100. Secondary Antibody: Red-fluorescent goat anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated ab96881 (2 different lots ) used at a dilution of 1/100 (5µg/mL). Counterstain: DAPI (blue)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (23)
ab96881 は 23 報の論文で使用されています。
- Wu N et al. Essential role of MALAT1 in reducing traumatic brain injury. Neural Regen Res 17:1776-1784 (2022). PubMed: 35017438
- Shi Y et al. ROS Promote Hypoxia-Induced Keratinocyte Epithelial-Mesenchymal Transition by Inducing SOX2 Expression and Subsequent Activation of Wnt/ß-Catenin. Oxid Med Cell Longev 2022:1084006 (2022). PubMed: 35035654
- Selinger M et al. Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors. Comput Struct Biotechnol J 20:2759-2777 (2022). PubMed: 35685361
- Selinger M et al. Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis. J Biol Chem 298:102585 (2022). PubMed: 36223838
- Yu M et al. TREM2 is associated with tumor immunity and implies poor prognosis in glioma. Front Immunol 13:1089266 (2022). PubMed: 36713360