Application
Western blot
5 ug of cell spermatocyte lysate with RIPA buffer
30 min skim milk blocking
Primary 1:2000 room temp for 1 hr
Secondary 1:2000 Room temp
Detection with Alkaline phosphatase substrate
30 min skim milk blocking
Primary 1:2000 room temp for 1 hr
Secondary 1:2000 Room temp
Detection with Alkaline phosphatase substrate
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投稿 Jul 06 2016