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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC)
IgG 抗体 (1691件) 一覧
Target species
Goat
Tested applications
IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
Immunogen
Full length native Goat IgG (purified).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Goat >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
ICC/IF: 1/1000 - 1/5000.
IM: Use at an assay dependent concentration.
Flow Cyt: 1/500 - 1/2500.
ELISA: 1/10000 - 1/50000.
Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC) images:
Flow Cytometry - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC) (ab6881)
Ab6881 was used at dilution 1/200 with the primary antibody ab27985 in Flow Cyt. See the review on ab27985.
Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC) (ab6881)
Ab6881 was used at dilution 1/500 with the primary antibody ab26234 in ICC/IF. See the review on ab26234.
References for Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC) (ab6881)
This product has been referenced in:
- Wu Wet al. Characterization of the interaction between human respiratory syncytial virus and the cell cycle in continuous cell culture and primary human airway epithelial cells. J Virol 85:10300-9 (2011).Read more (PubMed: 21795354) »
- Blancas AAet al. Endothelial Cells from Embryonic Stem Cells in a Chemically Defined Medium. Stem Cells Dev : (2011). Flow Cyt.Read more (PubMed: 21446878) »
See all 5 publications for this product
Publishing research using ab6881? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"