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Product Name
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Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP)
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP)
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Immunogen
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Goat IgG whole molecule
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions
WB: 1/5000 (from PubMed:17369188).
suitable for immunoblotting (dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Goat >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)の別名 |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Horseradish Peroxidase (HRP)
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Storage buffer
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Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, 0.01M Sodium phosphate, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
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This product has been used in: (two most recent references)
Pizzuto R et al. Pyruvate kinase in pig liver mitochondria. Arch Biochem Biophys 495:42-8 (2010). WB. PubMed: 20026031
Ahn YH et al. Electrophilic tuning of the chemoprotective natural product sulforaphane. Proc Natl Acad Sci U S A 107:9590-5 (2010). WB. PubMed: 20439747
See all 12 publication references for this product.
If you publish research using ab6741 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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