Anti-CGA/hCG alpha 抗体 [EP3373] (ab92738)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3373] to CGA/hCG alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-CGA/hCG alpha antibody [EP3373]
CGA/hCG alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3373] to CGA/hCG alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CGA/hCG alpha aa 1-100. The exact sequence is proprietary.
Database link: P01215 -
ポジティブ・コントロール
- WB: JAR whole cell lysate treated with Hydroxyurea (10mM 24h). IHC-P: Human placenta tissue.
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特記事項
This product has switched from a hybridoma to recombinant production method on 2nd August 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP3373 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92738の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000.
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IHC-P |
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000. |
IHC-P
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the glycoprotein hormones subunit alpha family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 1081 Human
- Omim: 118850 Human
- SwissProt: P01215 Human
- Unigene: 119689 Human
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別名
- Anterior pituitary glycoprotein hormones common subunit alpha antibody
- CG alpha antibody
- CG-alpha antibody
see all
画像
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All lanes : Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/2000 dilution
Lane 1 : JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate
Lane 2 : JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate treated with Hydroxyurea (10mM 24h)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CGA/hCG alpha with ab92738 at 1/600 (0.87 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab92738 は 1 報の論文で使用されています。
- Mathioudakis N et al. Expression of the pituitary stem/progenitor marker GFRa2 in human pituitary adenomas and normal pituitary. Pituitary 18:31-41 (2015). PubMed: 24402129