PE Anti-Glycophorin A 抗体 [JC159], prediluted (ab197142)
Key features and details
- PE Mouse monoclonal [JC159] to Glycophorin A, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
PE Anti-Glycophorin A antibody [JC159], prediluted
Glycophorin A 一次抗体 製品一覧 -
製品の詳細
PE Mouse monoclonal [JC159] to Glycophorin A, prediluted -
由来種
Mouse -
標識
PE. Ex: 488nm, Em: 575nm -
特異性
ab197142 does not react with Glycophorin B. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Glycophorin A. (Membrane preparation from splenic hairy cell leukemia).
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エピトープ
ab197142 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of Glycophorin A. -
ポジティブ・コントロール
- Flow Cyt: Human blood cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
ポリ/モノ
モノクローナル -
クローン名
JC159 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab197142の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
ターゲット情報
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機能
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). -
配列類似性
Belongs to the glycophorin A family. -
翻訳後修飾
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). -
細胞内局在
Cell membrane. Appears to be colocalized with SLC4A1. - Information by UniProt
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参照データベース
- Entrez Gene: 2993 Human
- Omim: 111300 Human
- SwissProt: P02724 Human
- Unigene: 434973 Human
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別名
- AI853584 antibody
- Blood group--MN locus antibody
- CD_antigen=CD235a antibody
see all
画像
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab197142 (JC159) PE antibody at 10 µL reagent/100 µL of peripheral whole blood.
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Separation of human erythrocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab197142 (JC159) PE antibody at 10 µL reagent/100 µL of peripheral whole blood.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab197142 は論文での使用が確認できていません。