Biotin Anti-Glycophorin A + B 抗体 [HIR2] (ab93548)
Key features and details
- Biotin Mouse monoclonal [HIR2] to Glycophorin A + B
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Biotin Anti-Glycophorin A + B antibody [HIR2]
Glycophorin A + B 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [HIR2] to Glycophorin A + B -
由来種
Mouse -
標識
Biotin -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
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ポジティブ・コントロール
- Human peripheral blood leukocytes
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特記事項
Mouse monoclonal [HIR2] to Glycophorin A (Biotin) also has a clone number GA-R2.Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% Gelatin -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
Binding of ab93548 to red cells at high antibody concentration causes cell agglutination. -
ポリ/モノ
モノクローナル -
クローン名
HIR2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93548の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt | (1) |
Use at an assay dependent concentration.
Use 0.125ug for 10^5 to 10^8 cells in a final volume of 100 ul. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. Use 0.125ug for 10^5 to 10^8 cells in a final volume of 100 ul. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
細胞内局在
Type I membrane protein. -
参照データベース
- Entrez Gene: 2993 Human
- Entrez Gene: 2994 Human
- SwissProt: P02724 Human
- SwissProt: P06028 Human
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別名
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (1)
ab93548 は 1 報の論文で使用されています。
- Chen G et al. [Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria] Zhonghua Xue Ye Xue Za Zhi 18:190-2 (1997). PubMed: 15622765