Anti-Glycogen synthase 1/GYS1 抗体 [EP817Y] (ab40810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]
Glycogen synthase 1/GYS1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra) or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Glycogen synthase 1/GYS1 aa 700-800 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa whole cell lysate (ab150035), HEK293 cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP817Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40810の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa). |
ターゲット情報
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機能
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
パスウェイ
Glycan biosynthesis; glycogen biosynthesis. -
関連疾患
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
配列類似性
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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参照データベース
- Entrez Gene: 2997 Human
- Entrez Gene: 14936 Mouse
- Entrez Gene: 690987 Rat
- Omim: 138570 Human
- SwissProt: P13807 Human
- SwissProt: Q9Z1E4 Mouse
- SwissProt: A2RRU1 Rat
- Unigene: 386225 Human
see all -
別名
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
see all
画像
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All lanes : Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/10000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Glycogen synthase 1/GYS1 knockout HeLa lysate
Lane 3 : HEK-293 lysate
Lane 4 : Daudi lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 81 kDaLanes 1-4: Merged signal (red and green). Green - ab40810 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.
ab40810 Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] was shown to specifically react with Glycogen synthase 1/GYS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265388 (knockout cell lysate ab257462) was used. Wild-type and Glycogen synthase 1/GYS1 knockout samples were subjected to SDS-PAGE. ab40810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab40810 は 19 報の論文で使用されています。
- Hu MM et al. Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food Nutr Res 66:N/A (2022). PubMed: 35382382
- Ma YD et al. The Effects of Altered Endometrial Glucose Homeostasis on Embryo Implantation in Type 2 Diabetic Mice. Reprod Sci 28:703-714 (2021). PubMed: 33151524
- Zheng L et al. High-Intensity Interval Training Restores Glycolipid Metabolism and Mitochondrial Function in Skeletal Muscle of Mice With Type 2 Diabetes. Front Endocrinol (Lausanne) 11:561 (2020). PubMed: 32922365
- Shen J et al. Pressure suppresses hepatocellular glycogen synthesis through activating the p53/Pten pathway. Mol Med Rep 19:5105-5114 (2019). PubMed: 31059076
- Kakhlon O et al. Guaiacol as a drug candidate for treating adult polyglucosan body disease. JCI Insight 3:N/A (2018). PubMed: 30185673