Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle-AT)
Loading amount
50 µg
Specification
Skeletal muscle-AT
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 38 kDa
Positive control
Lysates from mouse brain and heart
Additional data
Additional Notes
Lanes 1-8: Lysates from AT skeletal muscles.
Lane 9: Lysate from brain.
Lane 10: Lysate from heart.
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors
Lane 9: Lysate from brain.
Lane 10: Lysate from heart.
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. G.K Sakellariou
Verified customer
投稿 Sep 28 2012