Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (Mouse and Rat Hippocampus)
Specification
Mouse and Rat Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated Goat anti-rabbit IgG polyclonal (Da
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 100 kDa Non-specific: 36, 40, 42 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 22 2013