Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (ovary)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
50 µg
Specification
ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: ICL Goat anti-Rabbit IgG h+l
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 36, so many kDa
Additional data
Abcam response
Thank you for your valuable feedback. ab115730 has not been previously tested in Chinese Hamster sample. It is possible that this antibody may not be suitable for use in this species. To see whether the non-specific bands can be reduced by further protocol optimization, we have contacted the customer and provided suggestions. We still welcome other Abreviews to determine the suitability of using this antibody with Chinese Hamster sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Daniel Wang
Verified customer
投稿 Feb 24 2015