Anti-Glucose Transporter GLUT1 抗体 [EPR3915] (ab115730)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Glucose Transporter GLUT1 antibody [EPR3915]
Glucose Transporter GLUT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1 -
由来種
Rabbit -
特異性
We recommend not to boil the samples after lysis to get desired WB bands.
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アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: NIH/3T3, HepG2, HT-29, SW480, 3T3-L1 and PC-12 whole cell lysates. IHC-P: Rat kidney tissue; mouse liver tissue; human lung carcinoma, cervical carcinoma, colon carcinoma, liver, colon, kidney carcinoma, skeletal muscle, urinary bladder, heart and breast tissue. ICC/IF: HepG2 cells and A549 (SLC2A1 knockout A549 cells used as a negative control) cells. Flow Cyt (intra): HeLa and Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 7.70 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3915 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)
- HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195021)
- Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free (ab196357)
- Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
- PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
- Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
- APC Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab316298)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115730の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/40.
For unpurified, use 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
This product gave a positive signal in A549 (SLC2A1 knockout A549 cells used as a negative control) fixed with 100% methanol (5 min). |
WB | (11) |
1/100000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 54 kDa).
We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading. |
IHC-P | (4) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/40. For unpurified, use 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. This product gave a positive signal in A549 (SLC2A1 knockout A549 cells used as a negative control) fixed with 100% methanol (5 min). |
WB
1/100000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 54 kDa). We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. -
組織特異性
Expressed at variable levels in many human tissues. -
関連疾患
Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia. -
配列類似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 6513 Human
- Entrez Gene: 20525 Mouse
- Entrez Gene: 24778 Rat
- Omim: 138140 Human
- SwissProt: P11166 Human
- SwissProt: P17809 Mouse
- SwissProt: P11167 Rat
- Unigene: 473721 Human
see all -
別名
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
see all
画像
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All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/100000 dilution
Lane 1 : Wild-type HepG2 cell lysate
Lane 2 : LC2A1 knockout HepG2 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 mg/ml per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50-300 kDa why is the actual band size different from the predicted?Western blot: Anti-Glucose Transporter GLUT1 antibody [EPR3915] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab115730 was shown to bind specifically to Glucose Transporter GLUT1. A band was observed at 50-300 kDa in wild-type HepG2 cell lysates with no signal observed at this size in SLC2A1 knockout cell line ab280797 (knockout cell lysate ab284224). To generate this image, wild-type and SLC2A1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1 µg/ml
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 :Human SLC2A1 (Glucose Transporter GLUT1) knockout A549 cell line (ab261869)
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 kDaLanes 1 - 2: Merged signal (red and green). Green - ab196357 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab196357 was shown to recognize in wild-type A549 cells as signal was lost at the expected MW in SLC2A1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SLC2A1 knockout samples were subjected to SDS-PAGE. Ab196357 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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GLUT1 and GLUT3 are downregulated in KSHV-infected cells in human KS tumors
Representative illustration of dual immunofluorescence detection of LANA and GLUT1 or in a normal human skin section and a Karposi Sarcoma (KS) tumor section. Tissues were fixed with paraformaldehyhde and paraffin-embedded.
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Immunohistochemical expression of Glut1 in normal tongue epithelium and tongue cancer. Expression was greatest in lymphocytes (arrows in left upper and lower panels). In the normal oral epithelium, Glut1 was weakly expressed in the basal and spinous cells (left upper panel). In OSCC, Glut1 was upregulated, showing a level of expression comparable with lymphocytes (left and right lower panels). Scale bar, 100 μm.
Note: Glut1 = SLC2A (alternative names for the same target).
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All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/50000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates unboiled with 5% NFDM/TBST
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates boiled with 5% NFDM/TBST
Lane 3 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates unboiled with 5% NFDM/TBST
Lane 4 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates boiled with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?Exposure time
Lane 1 to 2: 10 seconds
Lane 3 to 4: 30 secondsWe recommend not to boil the samples after lysis to get desired WB bands.
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Immunohistochemical staining of paraffin embedded rat kidney with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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ab115730 staining SLC2A1 in wild-type A549 cells, with negative expression in SLC2A1 knockout A549 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab115730 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunofluorescence staining of HepG2 cells with purified ab115730 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab115730 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Alexa Fluor® 488 (ab195359) and Alexa Fluor® 647 (ab195020) conjugated versions are available for this clone.
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Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab115730 at a dilution of 1/40 (red line). The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Alexa Fluorr®488 (ab195359) and Alexa Fluorr®647 (ab195020) conjugated versions are available for this clone.
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Immunohistochemical staining of paraffin embedded mouse liver with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000000 dilution (purified)
Lane 1 : HepG2 whole cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HT-29 whole cell lysate
Lane 4 : SW480 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000 dilution (Unpurified)
Lane 1 : Jurkat lysate
Lane 2 : Mouse brain lysate
Lane 3 : Human fetal brain lysate
Lane 4 : 3T3L1 lysate
Lane 5 : Human fetal liver lysate
Lane 6 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa -
Overlay histogram showing HeLa cells stained with unpurified ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human colonic adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing positive staining in human normal liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing positive staining in human normal breast tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing positive staining in human normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing positive staining in human kidney carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing negative staining in human skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing positive staining in human urinary bladder transitional carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab115730 showing negative staining in human normal heart tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (235)
ab115730 は 235 報の論文で使用されています。
- Ji P et al. Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy. Mater Today Bio 18:100515 (2023). PubMed: 36582449
- Sato T et al. Enhanced glucose metabolism through activation of HIF-1α covers the energy demand in a rat embryonic heart primordium after heartbeat initiation. Sci Rep 12:74 (2022). PubMed: 34996938
- Ni WJ et al. Berberine regulates mesangial cell proliferation and cell cycle to attenuate diabetic nephropathy through the PI3K/Akt/AS160/GLUT1 signalling pathway. J Cell Mol Med 26:1144-1155 (2022). PubMed: 35001506
- Wu F et al. TGF-βRII regulates glucose metabolism in oral cancer-associated fibroblasts via promoting PKM2 nuclear translocation. Cell Death Discov 8:3 (2022). PubMed: 35013150
- Zhou X et al. BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis. Bioengineered 13:2471-2485 (2022). PubMed: 35068350