Application
Western blot
Sample
Human Purified protein (neuronal)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
75 µg
Specification
neuronal
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%fbs PBS-T
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 63 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 30 2012