Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.04 mU/well
製品の概要
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製品名
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
Glucose 6 Phosphate Dehydrogenase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Enzyme activity -
検出感度
> 0.04 mU/well -
全工程の試験時間
1h 00m -
製品の概要
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH or G6PD) in a variety of samples.
In the G6PD assay protocol, glucose 6 phosphate is oxidized, which leads to the conversion of a nearly colorless probe to an intensely colored product with an absorbance at 450nm.
The G6PD assay kit can detect as low as 0.04mU G6PD per well.
G6PD assay protocol summary:
- add samples and positive control to wells
- add reaction mix
- analyze on a microplate reader for 5-30 min -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K757 Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit. K757-100 is the same size as the 100 test size of ab102529.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer V 1 x 25ml Developer Solution III 1 vial Development Enzyme Mix IX 1 vial G6PDH Substrate 1 vial NADH Standard I 1 vial -
研究分野
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関連性
Glucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. -
別名
- EC=1.1.1.49
- G6PD
- G6PD1
see all
画像
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G6PDH Activity measured in mouse tissue lysates. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 1-3 fold.
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G6PDH Activity measured in cell lysates.
Samples with the concentration of 4e6 cells/mL were used. Samples were undiluted.
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G6PDH Activity measured in biological fluids. Samples were undiluted.
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G6PDH Activity measured in mouse tissue lysates. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were undiluted.
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G6PDH Activity measured in cell lysates.
Samples with the concentration of 4e6 cells/mL were used. Samples were undiluted.
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G6PDH Activity measured in biological fluids. Samples undiluted.
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Example of positive control and pork liver samples test curves obtained using ab102529
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Example of Standard curve obtained using ab102529
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab102529 は 20 報の論文で使用されています。
- Lin J et al. The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity. Oncogene 41:1024-1039 (2022). PubMed: 34997215
- Yang C et al. N-Acetylcysteine protects against cobalt chloride-induced endothelial dysfunction by enhancing glucose-6-phosphate dehydrogenase activity. FEBS Open Bio 12:1475-1488 (2022). PubMed: 35666067
- De Angelis M et al. Influenza Virus Down-Modulates G6PD Expression and Activity to Induce Oxidative Stress and Promote Its Replication. Front Cell Infect Microbiol 11:804976 (2021). PubMed: 35071051
- Matsuo K et al. Geranylgeranylacetone attenuates cerebral ischemia-reperfusion injury in rats through the augmentation of HSP 27 phosphorylation: a preliminary study. BMC Neurosci 22:9 (2021). PubMed: 33557752
- Trotta MC et al. Resolvin D1 reduces mitochondrial damage to photoreceptors of primary retinal cells exposed to high glucose. J Cell Physiol 235:4256-4267 (2020). PubMed: 31612492