Application
Western blot
Sample
Human Cell lysate - nuclear (Neurons)
Loading amount
20 µg
Specification
Neurons
Gel Running Conditions
Reduced Denaturing (8% PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal West Femto Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 97 kDa Non-specific: 135 kDa
Positive control
5 neuronal cells samples
Additional data
Additional Notes
Nitrocellulose Membrane, M-PER lysis buffer (Pierce), Protease Inhibitor Cocktail (Sigma). Lanes 1-5: human neuronal cells with increasing amounts of protein (5, 10, 15, 20, and 25 ug).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 26 2008