Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Cy3 a mouse (?
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500
Additional data
Additional Notes
Tissue fixation: 3-4h 4% buffered formaldehyde.
I also stained w/this ab 2h RT and than 2h secondary and the staining was nice.
Other channels:Dy488gpInsulin (Abcam) and TOTO3
I also stained w/this ab 2h RT and than 2h secondary and the staining was nice.
Other channels:Dy488gpInsulin (Abcam) and TOTO3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Miri Stolovich-Rain
Verified customer
投稿 Nov 09 2011