Anti-Glucagon Receptor 抗体 (ab109279)
Key features and details
- Rabbit polyclonal to Glucagon Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Glucagon Receptor antibody
Glucagon Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glucagon Receptor -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Glucagon Receptor aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129210) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: Caco2; HepG2; SW480.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109279の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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特記事項 |
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WB
1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
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機能
This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2642 Human
- Omim: 138033 Human
- SwissProt: P47871 Human
- Unigene: 208 Human
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別名
- Gcgr antibody
- GGR antibody
- GL R antibody
see all
画像
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All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 25 kDa, 51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 51 kDa could potentially be a cleaved form of Glucagon Receptor due to the presence of a 25 amino acid signal peptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab109279 は論文での使用が確認できていません。