製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Gata6
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide within residues: GAGAPVMTGA GESTNPENSE LKYSGQDGLY IGVSLASPAE VTSSVRPDSW, corresponding to internal sequence amino acids 540-589 of Human Gata6 (NP_005248)

  • ポジティブ・コントロール
    • MCF7 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Gata6 with ab86271 at 5ug/ml.

  • Anti-Gata6 antibody (ab86271) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,41 kDa. We are unsure as to the identity of these extra bands.
  • ab86271 staining Gata6 in human embryonic stem cells (H1 differentiated to HN4 positive hepatic progenitor cells) by Immunocytochemistry/ Immunofluorescence. Right image HNF4.

参考文献

ab86271 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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