製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Galectin 8
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthesized peptide derived from an internal region of human Galectin 8.

  • ポジティブ・コントロール
    • Extracts from 3T3 cells and human prostate carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects bands of approximately 36 and 60 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性
      Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
    • 配列類似性
      Contains 2 galectin domains.
    • ドメイン
      Contains two homologous but distinct carbohydrate-binding domains.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Gal 8 antibody
      • Gal-8 antibody
      • Gal8 antibody
      • Galectin-8 antibody
      • galectin-8g antibody
      • Lectin galactoside binding soluble 8 antibody
      • LEG8_HUMAN antibody
      • LGAL S8 antibody
      • Lgals8 antibody
      • PCTA 1 antibody
      • PCTA-1 antibody
      • PCTA1 antibody
      • Po66 carbohydrate binding protein antibody
      • Po66 carbohydrate-binding protein antibody
      • Po66 CBP antibody
      • Po66-CBP antibody
      • Prostate carcinoma tumor antigen 1 antibody
      see all

    画像

    • All lanes : Anti-Galectin 8 antibody (ab69631) at 1/500 dilution

      Lane 1 : extracts of 3T3 cells
      Lane 2 : extracts of 3T3 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using ab69631 at 1:50 dilution.
      Left image un-treated.
      Right image treated with immunizing peptide.

    参考文献

    This product has been referenced in:
    • Reticker-Flynn NE & Bhatia SN Aberrant glycosylation promotes lung cancer metastasis through adhesion to galectins in the metastatic niche. Cancer Discov 5:168-81 (2015). Read more (PubMed: 25421439) »
    • Reticker-Flynn NE  et al. A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis. Nat Commun 3:1122 (2012). WB, IHC ; Mouse . Read more (PubMed: 23047680) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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