Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cell line)
Loading amount
20 µg
Specification
Glioblastoma cell line
Treatment
Lysis buffer (urea/thiourea)
Gel Running Conditions
Reduced Denaturing (4-20% 1mm)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 23 kDa Non-specific: 35, 65, 170 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 05 2010