Anti-GST3 / GST pi 抗体 (ab53943)
Key features and details
- Goat polyclonal to GST3 / GST pi
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GST3 / GST pi antibody
GST3 / GST pi 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GST3 / GST pi -
由来種
Goat -
特異性
ab53943 is specific for GST pi. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) GST3/ GST pi (Rat)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.7% BSA -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
IgG fraction was prepared from the antisera using the caprylic acid precipitation method. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53943の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
配列類似性
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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参照データベース
- Entrez Gene: 2950 Human
- Omim: 134660 Human
- SwissProt: P09211 Human
- Unigene: 523836 Human
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別名
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
画像
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Amido Black stain and Western blot analysis of GST-π antibody.
Lane 1. Molecular weight marker.
Lane 2. Purified GST-π
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature. -
IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab53943 は 17 報の論文で使用されています。
- Zhang M et al. Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain. Nat Commun 12:5091 (2021). PubMed: 34429415
- Cheng Y et al. Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer. J Cancer 11:7302-7311 (2020). PubMed: 33193894
- Wu C et al. Long Noncoding RNA Plasmacytoma Variant Translocation 1 Regulates Cisplatin Resistance via miR-3619-5p/TBL1XR1 Axis in Gastric Cancer. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32407172
- Uzuncakmak TK et al. Comparison of the tissue expressions of glutathione S transferase isoenzymes among patients with morphea and healthy controls: A preliminary study. Dermatol Ther 33:e14363 (2020). PubMed: 33002252
- Cheng Y et al. Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells. Onco Targets Ther 12:11057-11068 (2019). PubMed: 31908475