Application
ELISA
Sample
Rat Purified protein (smooth muscle)
Specification
smooth muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: PBS-Tween20
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Calcium channel beta subunit was immobilzed on 96-well plate and increasing concentrations of GST-Cavalpha interaction domain (AID) was added. Bound GST-AID was detected using anti-GST-HRP conjugated antibody ab49923 using HRP reaction.
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投稿 May 06 2009