Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/3000
Detection
Detection method
ECL
Negative control
EV tranfected cells
Exposure
5 second(s)
Bands
Specific: ~30 kDa
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投稿 May 11 2018