Application
Western blot
Loading amount
150 µg
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS 0.1% Tween20
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: ECL anti-rabbit HRP linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 47 kDa Non-specific: 30 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
The specific molecular weight is expected at 47 kDa. A small band is observed with 150 ´g of protein loaded and the antibody diluted at 1:2000.
Observed band size : 30 kDa with 100 and 150´g of protein loaded.
We are unsure as the identity of this band.
Does not seem to work well in this experimental procedure and/or species
Abcam response
Many thanks for your valuable feedback. ab75814 has not been validated for use in this species, and it appears that under these experimental conditions, the product does not specifically detect GSK3 beta (phospho S9) in the mouse brain. We welcome further customer feedback. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Karine Thibault
Verified customer
投稿 Nov 10 2014