Anti-GSK3 (alpha + beta) 抗体 [EP933Y] (ab62368)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP933Y] to GSK3 (alpha + beta)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GSK3 (alpha + beta) antibody [EP933Y]
GSK3 (alpha + beta) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP933Y] to GSK3 (alpha + beta) -
由来種
Rabbit -
特異性
ab62368 detects both GSK3 alpha and GSK3 beta. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human GSK3 (alpha + beta). The exact sequence is proprietary.
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ポジティブ・コントロール
- 293T cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP933Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62368の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
特記事項 |
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Is unsuitable for Flow Cyt, ICC, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 2931 Human
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- Omim: 606784 Human
see all -
別名
- Factor A antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase 3 beta antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab62368 は 6 報の論文で使用されています。
- He Q et al. Mesenchymal stem cell-derived exosomes exert ameliorative effects in type 2 diabetes by improving hepatic glucose and lipid metabolism via enhancing autophagy. Stem Cell Res Ther 11:223 (2020). PubMed: 32513303
- Hsiao CH et al. Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm. Stem Cell Res Ther 10:270 (2019). PubMed: 31445515
- Liao P et al. Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis. Elife 6:N/A (2017). WB . PubMed: 28440748
- Li R et al. Akt SUMOylation regulates cell proliferation and tumorigenesis. Cancer Res 73:5742-53 (2013). PubMed: 23884910
- Weigand S et al. Global Quantitative Phosphoproteome Analysis of Human Tumor Xenografts Treated with a CD44 Antagonist. Cancer Res 72:4329-39 (2012). PubMed: 22777824
- Sundaresan NR et al. The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun. Nat Med 18:1643-50 (2012). WB ; Mouse . PubMed: 23086477