Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblast)
Specification
Fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS + 0.5 BSA + 0.05% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/400
Additional data
Additional Notes
Excellent staining of the ER tubules.
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Abcam user community
Verified customer
投稿 Nov 23 2011