Anti-GRP94 抗体 (ab18055)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: ICC/IF, WB
- Reacts with: Human, Monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GRP94 antibody
GRP94 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GRP94 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human, Monkey
非交差種: Mouse, Rat -
免疫原
Synthetic peptide (Human) (Internal sequence).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | ||
WB | (2) |
特記事項 |
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ICC: 1/100 - 1/400.
IF: 1/100 - 1/400.
WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
ターゲット情報
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機能
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
配列類似性
Belongs to the heat shock protein 90 family. -
細胞内局在
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 7184 Human
- Omim: 191175 Human
- SwissProt: P14625 Human
- Unigene: 192374 Human
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別名
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
画像
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Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.
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Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL. Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab18055 は 7 報の論文で使用されています。
- Li W et al. Blocking PERK resuces vascular smooth muscle cells from homocysteine-induced ER stress and apoptosis. Front Biosci (Landmark Ed) 25:536-548 (2020). PubMed: 31585902
- Marrugal Á et al. Impact of Heat Shock Protein 90 Inhibition on the Proteomic Profile of Lung Adenocarcinoma as Measured by Two-Dimensional Electrophoresis Coupled with Mass Spectrometry. Cells 8:N/A (2019). PubMed: 31370342
- Whitmore LC et al. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis. J Immunol 196:1376-86 (2016). WB, IP ; Human . PubMed: 26729809
- Kim N et al. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun 7:10631 (2016). PubMed: 26868272
- Weston CJ et al. Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein. J Neural Transm 120:951-61 (2013). ICC/IF ; Human . PubMed: 23474851
- Mulya A et al. Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1. J Lipid Res 51:514-24 (2010). ICC/IF ; Human . PubMed: 19767535
- Kang BY et al. Differential gene expression profiling in HELLP syndrome placentas. Reprod Sci 15:285-94 (2008). PubMed: 18421023