Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
LiCor buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: LiCor buffer
Secondary antibody
Name
Non-Abcam antibody was used: LiCor
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Odyssey scanner
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 30, 25, 15 kDa
Positive control
Human brain homogenate
Additional data
Abcam response
Customer could try diluting antibody further, shorter antibody incubation time or using another blocker to reduce the background (smaller non-specific bands with lower intensity ).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 24 2011