Anti-GluK1 抗体 (ab67316)
Key features and details
- Rabbit polyclonal to GluK1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-GluK1 antibody
GluK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GluK1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human GluK1 (C terminal).
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ポジティブ・コントロール
- P815 cells. Brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67316の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
ターゲット情報
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機能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. -
組織特異性
Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 14805 Mouse
- SwissProt: Q60934 Mouse
- Unigene: 5134 Mouse
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別名
- EAA3 antibody
- EEA3 antibody
- Excitatory amino acid receptor 3 antibody
see all
画像
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Immunofluorescence of GluK1 in Mouse Brain cells using ab67316 at 20 ug/ml.
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Paraffin-embedded mouse brain tissue stained for GluK1 using ab67316 at 5 µg/ml in immunohistochemical analysis.
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Lane 1 : Anti-GluK1 antibody (ab67316) at 0.5 µg/ml
Lane 2 : Anti-GluK1 antibody (ab67316) at 1 µg/ml
Lane 3 : Anti-GluK1 antibody (ab67316) at 2 µg/ml
All lanes : P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab67316 は 1 報の論文で使用されています。
- Domínguez-Sala E et al. Properties of the epileptiform activity in the cingulate cortex of a mouse model of LIS1 dysfunction. Brain Struct Funct 227:1599-1614 (2022). PubMed: 35103859