Application
ChIP on chip
Detection step
Microarray
Sample
Human Cell lysate - nuclear (Chip-Seq in MCF7 cells)
Specification
Chip-Seq in MCF7 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
Chip-seq of GREB1 was performed on asynchronous MCF7 cells crosslinked using formaldehyde. 10ug of DNA was used for each experiment. ChIP DNA was then extracted and amplified using Illumina sequencing adapters and DNA was sequenced.
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Secondary antibody
None used
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 12 2014