Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% serum, 0.1% triton, 0.1% BSA in PBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: donkey anti rabbit IgG Alexa fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Following fixation in 4% PFA, hESCs were assessed for GRB10 expression using 1:100 dilution of the primary antibody in 1% serum, 0.1% triton, 0.1% BSA in PBS followed by detection using a donkey anti rabbit IgG Alexa 488 (1:500). The results show positive expression (green) and nuclei stained blue using DAPI. However, the staining is a little diffuse hence average review.
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投稿 Apr 14 2016