Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 24°C
Secondary antibody
Name
Non-Abcam antibody was used: chicken anti goat Alexa Fluor 488
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
4% paraformaldehyde in 0.1M Phosphate Buffer, pH 7.4, was used to fix the tissue for 2 hours. Incubation of secondary antibody was 1 hour.
Abcam response
Thank you for submitting an Abreview and submitting feedback on this antibody in an untested application. Based on these results, this antibody may not be suitable for use in IHC-P.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Bridgette Chia
Verified customer
投稿 Jul 08 2010