Anti-LGR5 抗体 [EPR3065Y] (ab75850)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3065Y] to LGR5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LGR5 antibody [EPR3065Y]
LGR5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3065Y] to LGR5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Fetal skeletal, human and mouse skeletal, mouse and rat brain lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3065Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75850の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000. Predicted molecular weight: 100 kDa.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 100 kDa. |
ターゲット情報
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機能
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling. -
組織特異性
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. -
配列類似性
Belongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8549 Human
- Entrez Gene: 14160 Mouse
- Entrez Gene: 299802 Rat
- Omim: 606667 Human
- SwissProt: O75473 Human
- SwissProt: Q9Z1P4 Mouse
- Unigene: 658889 Human
- Unigene: 42103 Mouse
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別名
- FEX antibody
- G protein coupled receptor 49 antibody
- G protein coupled receptor 67 antibody
see all
画像
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Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified) + Human skeletal muscle lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaBlocking Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified)
Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Rat skeletal muscle lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.We are unsure about the identity of the additional band.
Blocking Buffer and concentration: 5% NFDM/TBST
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Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (unpurified) + Fetal skeletal muscle tissue lysate at 10 µg
Secondary
HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (52)
ab75850 は 52 報の論文で使用されています。
- Chiang HY et al. IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. Nat Commun 13:874 (2022). PubMed: 35169117
- Chang ZY et al. Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269806
- Choi KM et al. The interferon-inducible protein viperin controls cancer metabolic reprogramming to enhance cancer progression. J Clin Invest 132:N/A (2022). PubMed: 36227691
- Chamseddine D et al. The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response. Cell Rep 41:111789 (2022). PubMed: 36516750
- Dunbar K et al. Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1. Cell Mol Gastroenterol Hepatol 11:465-489 (2021). PubMed: 32971322