製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GKAP1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between N terminal amino acids 1-50 of Human GKAP1 (NP_079487.2).

  • ポジティブ・コントロール
    • 293T, HeLa and Jurkat whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab123594 was affinity purified using an epitope specific to GKAP1 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 配列類似性
    Belongs to the GKAP1 family.
  • 細胞内局在
    Golgi apparatus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cGMP dependent protein kinase anchoring protein 42kDa antibody
    • cGMP dependent protein kinase anchoring protein of 42 kDa antibody
    • cGMP-dependent protein kinase-anchoring protein of 42 kDa antibody
    • FKSG21 antibody
    • G kinase anchoring protein 1 antibody
    • G kinase-anchoring protein 1 antibody
    • gkap1 antibody
    • GKAP1_HUMAN antibody
    • GKAP42 antibody
    • Protein kinase anchoring protein GKAP42 antibody
    see all

画像

  • All lanes : Anti-GKAP1 antibody (ab123594) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Exposure time : 10 seconds
  • Detection of GKAP1 by Western Blot of Immunprecipitate.
    ab123594 at 1µg/ml staining GKAP1 in 293T whole cell lysate immunoprecipitated using ab123594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

ab123594 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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