Anti-GFPT1 抗体 [EPR4854] (ab125069)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4854] to GFPT1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GFPT1 antibody [EPR4854]
GFPT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4854] to GFPT1 -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human GFPT1 aa 600-700. The exact sequence is proprietary.
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ポジティブ・コントロール
- IHC-P: Human testis, and Human breast carcinoma tissue; WB: Human placenta lysate, MCF7, C6, MEF, 293T, JAR and HeLa cell line lysates, Mouse heart and Mouse cerebral cortex, Rat heart tissue lysate. IP: Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4854 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125069の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/200. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
For unpurified use at 1/1000 - 1/1000. |
IP |
1/10 - 1/100.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
特記事項 |
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WB
1/200. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). For unpurified use at 1/1000 - 1/1000. |
IP
1/10 - 1/100. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ターゲット情報
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機能
Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. -
組織特異性
Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle. -
パスウェイ
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1. -
関連疾患
Defects in GFPT1 are the cause of limb-girdle myasthenia with tubular aggregates (LGMTA) [MIM:610542]. A congenital myasthenic syndrome characterized by onset of proximal muscle weakness in the first decade. Individuals with this condition have a recognizable pattern of weakness of shoulder and pelvic girdle muscles, and sparing of ocular or facial muscles. EMG classically shows a decremental response to repeated nerve stimulation, a sign of neuromuscular junction dysfunction. Affected individuals show a favorable response to acetylcholinesterase (AChE) inhibitors. -
配列類似性
Contains 1 glutamine amidotransferase type-2 domain.
Contains 2 SIS domains. - Information by UniProt
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参照データベース
- Entrez Gene: 2673 Human
- Entrez Gene: 14583 Mouse
- Entrez Gene: 297417 Rat
- Omim: 138292 Human
- SwissProt: Q06210 Human
- SwissProt: P47856 Mouse
- SwissProt: P82808 Rat
- Unigene: 580300 Human
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別名
- CMS12 antibody
- CMSTA1 antibody
- D-fructose-6-phosphate amidotransferase 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GFPT1 with Purified ab125069 at 1:1000 dilution (2.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab125069 (purified) at 1:100 dilution (2µg) immunoprecipitating GFPT1 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab125069 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125069 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/200 dilution (Purified) + C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa -
All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse cerebral cortex lysates
Lane 4 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates
Lane 5 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaThis antibody is not suitable to detect tissue lysate samples.
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ab125069, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : JAR cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 79 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab125069 は 22 報の論文で使用されています。
- Dragic H et al. The hexosamine pathway and coat complex II promote malignant adaptation to nutrient scarcity. Life Sci Alliance 5:N/A (2022). PubMed: 35396334
- Wei S et al. GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. Cell Discov 8:77 (2022). PubMed: 35945223
- Li ML et al. The RNA-binding protein AUF1 facilitates Akt phosphorylation at the membrane. J Biol Chem 298:102437 (2022). PubMed: 36041631
- Ruegenberg S et al. Protein kinase A controls the hexosamine pathway by tuning the feedback inhibition of GFAT-1. Nat Commun 12:2176 (2021). PubMed: 33846315
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873