Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti-Chicken IgY (H+L) Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Embryonic hearts dissected and fixed in 0.2% PFA at 4'C O/N prior to embedding in OCT.
Sections (10um thick) were subjected to 4% PFA fix on slide for 5mins. Sections were blocked using 5% Secondary specific serum + 0.5% Tween-20 + 1% BSA for 2hrs at RT.
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投稿 Apr 06 2017