Application
Immunoprecipitation
Sample
Human Cell lysate - other (HEK 293)
Total protein in input
300 µg
Specification
HEK 293
Treatment
Transfected with CFP-fused protein XXX in pECFP vector
Immuno-precipitation step
Protein G
Other product details
Concentration
6.7 µg/mg lysate
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS + detergent + protease inhibitors
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
ab291 rapidly bound CFP (depleted from 'unbound' fraction compared to total lysate) allowing IP of CFP-fused protein XXX in as little as 30 min. 2ug captured almost all of the target protein from only 300ug total protein in an overnight IP: a lot of ab291 antibody may be required for complete IP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Lindsay Tulloch
Verified customer
投稿 Dec 10 2009