Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/30000
Secondary antibody
Name
Non-Abcam antibody was used: Pierce 31210
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 40 kDa
Exposure
5 second(s)
Positive control
HeLa cells were transfected with a 15 kDa protein tagged with GFP. The cells were lysed and 10 (lanes 3 and 4) or 20 ´g (lanes 1 and 2) of the WEC was run on a SDS-PAGE gel.
Additional data
Additional Notes
HeLa cells were transfected with a 15 kDa protein tagged with GFP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 19 2013