Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate pH 6.0
Permeabilization
Yes - Triton 0.2%
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 20%
Other product details
Dilution
1/50
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% serum, 0.2% Triton in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Streptavidin-Cy2 Jackson
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
The rat's brains were infected with viruses encoding GFP under a CMV promoter. Fixation was carried out by perfusion with 2.5% PFA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Efrat Shema
Verified customer
投稿 Aug 02 2011