Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cells overexpressing a GFP fusion protein)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
CHO cells overexpressing a GFP fusion protein
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + tweem 20
Dilution
1/5000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 55 and 30 kDa
Positive control
The ab recognized the fusion protein of 55 kDa as well as the 30 kDa band corresponding to GFP probably from degradation of the fusion protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 25 2016