Anti-GFM1 抗体 [EPR12263] (ab171945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12263] to GFM1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GFM1 antibody [EPR12263]
GFM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12263] to GFM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment within Human GFM1. The exact sequence is proprietary.
Database link: Q96RP9 -
ポジティブ・コントロール
- HeLa, NCIH460, U87 MG and 293T cell lysates; Human cervical carcinoma tissue and Human kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12263 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171945の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. -
パスウェイ
Protein biosynthesis; polypeptide chain elongation. -
関連疾患
Defects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy. -
配列類似性
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 85476 Human
- Omim: 606639 Human
- SwissProt: Q96RP9 Human
- Unigene: 518355 Human
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別名
- COXPD1 antibody
- EF-Gmt antibody
- EFG antibody
see all
画像
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All lanes : Anti-GFM1 antibody [EPR12263] (ab171945) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NCI 460 cell line lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling GFM1 with ab171945 at 1/50
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling GFM1 with ab171945 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab171945 は 1 報の論文で使用されています。
- Simon MT et al. Activation of a cryptic splice site in the mitochondrial elongation factor GFM1 causes combined OXPHOS deficiency. Mitochondrion 34:84-90 (2017). PubMed: 28216230