Application
ChIP
Sample
Mouse Cell lysate - nuclear (Hematopoietic progenitors)
Specification
Hematopoietic progenitors
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Detection step
Real-time PCR
Positive control
GFI-1 locus
Negative control
Alpha actin locus
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP buffer
Additional data
Abcam response
Thank you for your honest feedback. This antibody has never been tested in ChIP, so it may in fact not be suitable for this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 03 2009